Next-generation sequencing · Vienna, Austria

Send us your samples. Get back answers you can publish. Guaranteed.

QC, library prep, sequencing and custom bioinformatics in one project. You get figures, interpretation and a written Methods section.

  • Free study-design review
  • Sample kit and courier paid by us
  • Complications fixed at €0
sample S07 · stool · 0.6 ng/µLQC → sequencing → analysis
From sequencing reads to a finished figure Short sequencing reads align to a reference and build up coverage, then become a publication figure and a Materials and Methods paragraph. READS reference COVERAGE ANALYSIS ↓ Fig. 2differential abundancelog2 fold change Materials & MethodsReads were quality-filteredand denoised with DADA2(QIIME 2). Taxonomy wasassigned against SILVA 138.1.Differential abundance wastested with ANCOM-BC andALDEx2 (BH-adjusted q < 0.05).
  • 5.6%of samples needed re-sequencing. We paid for every one.
  • €0for failed libraries, low depth or reviewer re-analysis
  • <1ng/µLis routine input for most samples we receive
  • 3platforms: Illumina, Oxford Nanopore and PacBio
Why NGS Analytics

Sequencing is the easy part. We take care of the rest.

The usual provider you coordinate

  • You pick the method and depth alone
  • Shipping and customs are your problem
  • Failed runs are billed to you
  • You get a folder of FASTQ files
  • Reviewer questions mean a new quote

NGS Analytics one team, one quote

  • A scientist reviews your design first
  • Kit, courier and customs paid by us
  • Complications fixed at €0
  • Figures, interpretation and a Methods section
  • Re-analysis for reviewers included
How it works

Four steps. You only do the first one.

  1. Labelled sample tubes stored in racks in a laboratory freezer

    Send your samples

    Email us the question. You get a design review, a fixed quote, a kit and a paid courier.

  2. Gloved hands pipetting samples into a tube rack next to a centrifuge

    QC and library prep

    Every sample is checked first. You see the QC result before library prep starts.

  3. Two flow cells loaded on the stage of an Illumina NovaSeq X Plus sequencer

    Sequencing

    Illumina NovaSeq X Plus, Oxford Nanopore or PacBio, whichever fits your question.

  4. Volcano plot of differentially expressed genes, up-regulated in teal and down-regulated in coral

    Answers you can publish

    Figures, interpretation, a Methods section and a follow-up call.

What you receive

A result, not a folder of files.

  1. Draft figure from the first round: Shannon diversity in a control group and two dose groups, labelled with the column names from the sample sheet

    Raw data and a first analysis

    We start from your sequencing data and the plan we agreed in the design call: your groups, your comparisons, your question.

  2. round 010203 Use our group names, and colour themAdd the statisticsPerfect. That one goes in the paper

    Rounds of changes with you

    You look at the figures and tables and tell us what to change. We rerun the analysis and send them back.

    As many rounds as you want
  3. The finished figure sitting on a manuscript page, cut out of our report Fig. 2 | Diversity by dose

    Figures you can paste into the paper

    The last round ends with publication-ready figures, tables and the matching Methods text. Take them out of the report and into your manuscript.

  • Interpretation report
  • Publication-ready figures
  • Materials & Methods
  • Results tables
  • Raw and processed data
The 100% Complete Project Guarantee

If something goes wrong, you pay €0 to fix it.

About 5.6% of samples need re-sequencing. Every one of those re-runs is paid by us, not by the researcher.

  • No arguments
  • No disputes
  • No unexpected invoices
The invoice you won’t receive
Complications during your project
Covered
  • Sample quality problemsWe troubleshoot, re-extract and re-ship€0.00
  • Library prep failureFull redo€0.00
  • Not enough sequencing depthImmediate resequencing€0.00
  • Reviewer wants a different analysisCustom re-analysis€0.00
  • Anything elseWe solve it. No arguments.€0.00
Amount due€0.00

Covered by the 100% Complete Project Guarantee, in every project.

Numbered sample tubes from a field collection in a rack
Field samples
Blood collection tubes with coloured caps
Blood
Gloved hand holding a Petri dish with bacterial colonies
Isolates
Numbered 96-well plate with samples
96-well plates
Difficult samples

Low input, FFPE, low biomass: send them anyway.

Most samples we receive are below 1 ng/µL, and we sequence them.

  • Low inputBelow 1 ng/µL is routine
  • FFPEBlocks and slides, including tumour
  • Low biomassRun with blanks and controls
  • DegradedAncient-DNA-style extraction
  • Stool
  • Soil
  • Sediment
  • Seawater
  • FFPE blocks
  • Brain cortex
  • Blood
  • Swabs
  • Ticks
  • Insects
  • Shrimp
  • Horse
  • Plant leaves
  • Yeast
  • Sourdough
  • Sand
  • Cleanroom swabs
  • and more…
The guarantee in practice

What “whatever it takes” looks like.

  • Genome project · Poland

    Extraction below spec, project upgraded

    The first isolation didn’t yield enough high-molecular-weight DNA. We re-isolated for free and moved the project to PacBio HiFi, absorbing the difference.

    Extra cost to the lab€0
  • Low-DNA samples · Brazil

    Too dilute to measure, sequenced anyway

    The DNA sat below what a fluorometer can read. We ran our low-input recovery workflow and sequenced the samples at no charge.

    Extra cost to the lab€0
  • Microbiome study · Bologna

    Extra extractions, kept off the bill

    Several samples needed another extraction before library prep. We ran them and left them off the invoice.

    Extra cost to the lab€0

More projects we finished

Questions

Straight answers before you email.

Anything else: info@ngsalytics.com

What does a project cost?

Every project gets a fixed quote after the free design review. It covers sequencing, analysis, figures, the Methods text and the guarantee, so the quote is the invoice.

My samples are low input or degraded. Should I send them?

Yes. Most samples we receive are below 1 ng/µL, and our recovery pipelines save samples other labs discard.

Which sequencing platform will you use?

The one that fits your question. We run projects on Illumina, Oxford Nanopore and PacBio, and often price the same project on two platforms side by side.

Do I need a bioinformatician?

No. Custom analysis, figures and the Methods text are part of every project. If you have one, they get all results as tables and the processed data to work with.

Can you work with human and clinical samples?

Yes. We are an EU company, work under GDPR and sign a data processing agreement before any data moves.

I already have data. Can you only do the analysis?

Yes. Bioinformatics only covers data you already have, from any provider or public archive.

Start here

Tell us about your samples.

One email starts the project. A scientist reads it and replies.

Email us about your project

What to put in your first email

  1. What your samples are, and how many you have
  2. The question you want the data to answer
  3. Your deadline: a paper, a grant or a reviewer
  4. Anything unusual: low input, FFPE, low biomass